anced Induction of Mucin - Depleted Foci in Estrogen Pr R eptor β Knockout Mice
نویسندگان
چکیده
wnloaded role of the estrogen receptor β (ERβ) in the colon has received considerable interest, yet in vivo ls are needed to better define its protective actions. In the present study, wild-type (WT), ERα, Rβ knockout (αERKO and βERKO) mice were injected with azoxymethane, a colon chemical carn. Fourteen weeks after azoxymethane exposure, the incidence of aberrant crypt foci (ACF) was ed by methylene blue staining. βERKO mice showed significantly higher incidence (P < 0.001) F (15.0 ± 2.5) compared with αERKO (3.4 ± 1.0) and WT (4.6 ± 1.0) mice. The colons in several O mice had increased thickness and loss of normal morphology. It has been reported that ERβ plays in the maintenance of the colonic crypt architecture; this may explain the loss of crypt organization colonic epithelium of βERKO mice. The presence of mucin-depleted foci (MDF) has been shown, n humans and in rodents, as an early event in colon cancer. Therefore, to surpass the limitations CF scoring, we performed Alcian blue-neutral red staining to assess the presence of MDF. This assay d the assessment of precancerous lesions on all the βERKO mice colons (38.3 ± 4.0; P < 0.001), aring to WT and αERKO mice (6.6 ± 1.5 and 10.0 ± 1.9, respectively), and served to confirm the ACF comp results. Together, these data support the use of MDF staining as a biomarker for precancerous lesions and the protective role of ERβ in colon carcinogenesis. Cancer Prev Res; 3(9); 1198–204. ©2010 AACR.
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تاریخ انتشار 2010